InovaChrom employs a unique and diverse set of separation technologies that allow us to solve complex and difficult purification problems. Our technologies have evolved from our extensive natural products purification experience but are broadly applicable to most small molecules from all sources. The proper combination of technologies can provide cost effective and scalable solutions for purification problems. Our separation processes are designed for efficient scale up.

Countercurrent separation

Countercurrent Chromatography

Countercurrent Chromatography

Countercurrent separation (CCS), also known as countercurrent chromatography (CCC), is pure liquid-liquid chromatography without a solid support.  The “column” for CCS is simply a biphasic mixture of two or more solvents.  InovaChrom has extensive expertise in determining the correct mixture of solvents required for CCS.

Advantages of CCS include:

  • Selectivity which complements that of Reverse Phase (RP) HPLC and SFC
  • Complete recovery of injected sample
  • Lower solvent consumption than conventional chromatography
  • No costly solid stationary phases

Macroporous Resin Chromatography, Ion Exchange, and Medium Pressure Chromatography

InovaChrom utilizes a wide range of separation methods to complement our CCS technology. These range from classical silica gel column chromatography to modern macroporous resin separations.

Polymeric macroporous resin adsorption/elution and chromatography have many advantages for complex purification problems. These resins are available in various neutral polymers and ion exchangers and have long been a mainstay for natural product purification.